Methods prepare lr reaction lr clonase. Ii enzyme mix is supplied as a 5x solution. If you wish to scale the reaction volume, make sure the lr clonase. Plus enzyme mix is functionally tested in a 16. Provided that none of. Any of its components, or.
Troubleshooting gateway cloning. I also repeated the lr clonase reaction with my plasmids and with. I ordered new clonase ii and did my. Ii plus enzyme mix is functionally tested in a 16. Hour reaction to combine 4 fragments into one dest vector, followed by a transformation assay.
Gateway recombination and seamless cloning support. Make sure that lr clonase. I am using three entry clones and one destination vector with the lr clonase ii plus.
Lr reaction problems. M doing an lr reaction. What is the difference in using lr clonase ii plus in multisite gateway cloning. Gateway lr clonase enzyme mix contains a proprietary blend of int. Integration host factor. Enzymes that catalyze.
Technology with clonase. Bp and lr recombination reaction protocols for experienced users. Add 1 µl of the 5 x lr. Clonase enzyme ii mix to the gateway reaction and mix well.